Ihe ngwụcha Chromogenic Kit EC64405

Ngwa nyocha Bioendo EC Endotoxin na-enye ụdị nleta maka ngwọta nnwale endotoxin ọnụọgụ.Ihe endotoxin dị na nlele ahụ na-eme ka oghere nke enzymes dị na Amebocyte Lysate, enzyme na-arụ ọrụ na-ekewa mkpụrụ nke sịntetik, na-ahapụ ngwaahịa na-acha odo odo na ntinye kachasị na 405nm.Maka ngwụcha ngwụcha Chromogenic Endotoxin Kit na-enweghị njikọta Diazo, achọrọ spectrophotometer ma ọ bụ onye na-agụ microplate mgbe niile ka ọ gụọ nnabata ahụ na 405nm.Igwe nleba anya mgbe niile nwere ọrụ nke nnwale kinetic, izi ezi nke njikwa okpomọkụ na nzacha 405nm, wee jiri spectrophotometer ahụ gụọ nsonaazụ endotoxin nke EC kit.


Nkọwa ngwaahịa

Ngwa nyocha Chromogenic Endotoxin ngwụcha (na-enweghị njikọ Diazo)

1. Ngwaahịa Ozi

Ngwa nyocha Chromogenic Endotoxin nke ngwụchaA na-eduzi (na-enweghị Diazo Coupling) site n'ịgbakwunye ihe ngwọta peptide na-enweghị ụcha n'ime ngwakọta nke Amebocyte Lysate Lyophilized na nwalee ihe nlele mgbe oge ụfọdụ gasịrị.Ọ bụrụ na nlele ule nwere endotoxin, agba odo ga-etolite na microplate 96 nke ọma.Ntinye ya (λmax = 405nm) metụtara mkpokọta endotoxin.Enwere ike gbakọọ mkpokọta endotoxin nke nlele ule dabere na usoro ọkọlọtọ.

2. Nhọrọ ngwaahịa

Oke mmetụta uche: 0.01-0.1 EU/ml, oge nyocha dabara bụ ihe dị ka nkeji 46

0.1-1 EU/ml, oge nyocha dabara na ihe dịka nkeji iri na isii.

3. Njirimara ngwaahịa na ngwa

A na-ezubere Kit Test Chromogenic Endotoxin ngwụcha (na-enweghị Diazo Coupling) maka iji na nchọpụta In Vitro na ọnụọgụ nke endotoxins nje gram-adịghị mma.A na-agbakwunye ihe ngwọta peptide artificial na-enweghị agba na ngwakọta nke Lyophilized Amebocyte Lysate ma nwalee ihe nlele mgbe oge ụfọdụ gasịrị.Agba odo na-etolite ma ọ bụrụ na nlele ule nwere endotoxin.Mgbe ahụ, jiri spectrophotometer ma ọ bụ ihe na-agụ efere mgbe niile ka ịgụọ ihe na-amị amị na 405nm.Ngwunye ule Chromogenic Endotoxin nke ngwụcha (na-enweghị Diazo Coupling) dabara maka ụdị nlele ahụike ahụike dị ka protein, ọgwụ mgbochi, plasmid, DNA, RNA endotoxin assay.

Mara:

Lyophilized Amebocyte Lysate (LAL) reagent nke Bioendo rụpụtara bụ nke sitere na amebocyte lysate enwetara ọbara nke nshịkọ akpụkpọ ụkwụ.

Katalọgụ No.

Nkọwa

Ọdịnaya ngwa

Mmetụta

(EU/ml)

EC64405

 

Ngwa nyocha Bioendo™ EC Endotoxin (Nchọpụta Chromogenic nke ngwụcha),

64 Nnwale/Kit

 

2 Lyophilized Amebocyte Lysate, 1.7ml / vial;

2 mmiri maka BET, 50ml / vial;

2 CSE;

4 Chromogenic mkpụrụ, 1.7ml/vial;

0.1-1 EU / ml

EC64405S

0.01 - 0.1 EU / ml;

0.1 - 1 EU/m

Ọnọdụ ngwaahịa:

A na-enyocha uche nke Lyophilized Amebocyte Lysate na ike nke Control Standard Endotoxin megide USP Reference Standard Endotoxin.Ngwa Lyophilized Amebocyte Lysate reagent ngwa na-abịa na ntuziaka ngwaahịa, Asambodo nyocha.

 

Ngwa njedebe endotoxin ọ chọrọ onye na-agụ microplate ọkaibe?

Bioendo EC64405 na EC64405S kit na-enyocha nsonaazụ endotoxin ọnụọgụ site na onye na-agụ microplate mgbe niile.

Usoro ihe nyocha chromogenic endotoxin njedebe:

Endotoxin Free tube

Ndụmọdụ na-enweghị Pyrogen

Pyrogen Free Microplates

Onye na-agụ Microplate mgbe niile

Ọkọlọtọ njikwa dakọtara endotoxin (CSE10V)

Iji mmiri nzọ nrụzigharị.

 



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