Ngwa mwepụ EtEraser™ SE Endotoxin
Ngwa mwepụ EtEraser™ SE Endotoxin
1. Ngwaahịa ozi
Lipopolysaccharide (LPS) bụ endotoxin na-akpata nje na ihe bụ isi nke mgbidi cell nke nje bacteria gramnegative.Protein recombinant sitere na E.coli na-enwekarị endotoxins dị elu.Mwepụ nke endotoxins dị oke mkpa maka usoro mgbada.
Ngwa mwepụ EtEraser SE Endotoxinemebere iji wepụ mmetọ endotoxin na ngwọta mmiri.Ngwa ndị ahụ nwere endotoxin mwepụ resin na-ejikọta iji belata ọkwa endotoxin na nlele protein site na≥99% n'ihe na-erughị awa 2.
Ngwa ahụ gụnyere kọlụm mwepụ endotoxin eburula ụzọ jupụta 1.5 ml, ihe nchekwa ihe ndozi, ihe nchekwa nrụpụta na tube mkpokọta enweghị pyrogen na ndụmọdụ.Kọlụm ahụ nwere ikike ijikọ dị elu nke> 2,000,000 EU / ml.Enwere ike ijikwa ngwaahịa a ugboro ise ma ọ bụrụ na emegharịrị nke ọma.The affinity resin dị na slurry na nwere ike ịbụ elu-scalingin biopharmaceutical usoro.
Ngwa mwepụ EtEraser SE Endotoxinna-eji ọkwa nri gbanwetụrụε-poly-L-lysineligand ashigh affinity ligand maka endotoxins.Resin bụ beads agarose nke kwụsiri ike na enweghị mmetụta cytotoxic n'ahụ mmadụ.Mgbe emechara ka ọ dị ọcha, ọ nweghị ihe fọdụrụ na-egbu egbu fọdụrụ na nlele.Nke angwa mwepụ nke endotoxinenwere ike iji ya na ọgwụ protein, ọgwụ mgbochi, ọgwụ mgbochi, DNA/RNA, polysaccharide na ihe atụ ndị ọzọ.
2. Njirimara ngwaahịa
• nkwụsi ike dị elu - anaghị emetụta ọrụ nke ọtụtụ n'ime ihe atụ nke usoro ndu
• ikike njikọ dị elu -> 2000000 EU / ml
• anaghị egbu egbu —–> ligands nri anaghị egbu egbu, enweghị mmetụta cytotoxic nke poly lysine.
• mgbake protein dị elu -> 95% mgbake protein maka ụdị protein
• elu mwepụ arụmọrụ - wepụ> 99% endotoxin, endotoxin larịị na sample nwere ike na-erughị 0.1 EU / ml mgbe endotoxin wepụrụ usoro.
• oke ngwa ngwa - enwere ike iji na mwepụ endotoxin maka protein, peptides, ọgwụ mgbochi, ọgwụ mgbochi, polysaccharide na ihe nlele ndị ọzọ.