Kedu ihe bụ Test Endotoxins?

Kedu ihe bụ Test Endotoxins?

Endotoxins bụ ụmụ irighiri mmiri hydrophobic bụ akụkụ nke lipopolysaccharide complex nke na-etolite ọtụtụ n'ime mpụta nke nje bacteria Gram-negative.A na-ahapụ ha mgbe nje bacteria na-anwụ ma akpụkpọ ahụ ha dị n'èzí gbasasịrị.A na-ewere endotoxins dị ka ndị bụ isi na-enye aka na nzaghachi pyrogenic.Na ngwaahịa parenteral emeru ya na pyrogens nwere ike ibute mmepe nke ahụ ọkụ, ntinye nke nzaghachi mkpali, ujo, ọdịda akụkụ ahụ na ọnwụ n'ime mmadụ.

Nnwale Endotoxins bụ ule iji chọpụta ma ọ bụ tụọ ọnụọgụ endotoxins sitere na nje bacteria Gram-negative.

A na-eji oke bekee arụ ọrụ iji chọpụta ma chọpụta endotoxins na ngwaahịa ọgwụ na mbụ.Dị ka USP si kwuo, RPT gụnyere nlekota maka mmụba nke okpomọkụ ma ọ bụ ahụ ọkụ mgbe ịgbanye ọgwụ n'ime eriri afọ nke ọgwụ n'ime oke bekee.Na 21 CFR 610.13(b) chọrọ nnwale pyrogen oke bekee maka ngwaahịa ndu akọwapụtara.

N'afọ 1960, Fredrick Bang na Jack Levin chọpụtara na amebocytes nke nshịkọ akpụkpọ ụkwụ ga-agbakọta n'ihu endotoxins.NkeLimulus Amebocyte Lysate(ma ọ bụ Tachypleus Amebocyte Lysate) ka emepụtara ya iji dochie ọtụtụ RPT.Na USP, a na-akpọ ule LAL dị ka ule endotoxin nke nje bacteria (BET).Enwere ike ime BET site na iji usoro atọ: 1) usoro gel-clot;2) usoro turbidimetric;3) Usoro chromogenic.Ihe achọrọ maka ule LAL nwere pH kacha mma, ike ionic, okpomọkụ, na oge nnabata.

E jiri ya tụnyere RPT, BET na-adị ngwa ngwa ma na-arụ ọrụ nke ọma.Agbanyeghị, BET enweghị ike dochie RPT kpamkpam.N'ihi na nyocha LAL nwere ike igbochi ya site na ihe na-enweghị ike ịchọpụta pyrogens na-abụghị endotoxin.