Endotoxins bụ obere ụmụ irighiri mmiri hydrophobic lipopolysaccharides (LPS) sitere na nje bacteria na-achọta n'ime mpụta sel nke nje bacteria gram-adịghị mma.Endotoxins nwere agbụ polysaccharide isi, O-kpọmkwem polysaccharide akụkụ (O-antigen) na lipid compenent, Lipid A, nke na-ahụ maka mmetụta nsị.Bacteria na-awụfu endotoxin buru ibu mgbe mkpụrụ ndụ nwụrụ na mgbe ha na-eto eto ma na-ekewa.Otu Escherichia coli nwere ihe dị ka nde molekul LPS n'otu sel.
Endotoxin nwere ike imerụ labwares n'ụzọ dị mfe, ọnụnọ ya nwere ike inye aka nke ukwuu ma nnwale na vitro na vivo.Na maka ngwaahịa parenteral, ngwaahịa parenteral nke emeru na endotoxins gụnyere LPS nwere ike ibute mmepe nke ahụ ọkụ, ntinye nke nzaghachi mkpali, ujo, ọdịda akụkụ ahụ na ọnwụ n'ime mmadụ.Maka ngwaahịa dialysis, enwere ike ibufe LPS site na akpụkpọ ahụ nwere nnukwu pore site na nzacha azụ site na mmiri dialysis gaa n'ọbara, enwere ike ịkpata nsogbu mkpali.
A na-achọpụta Endotoxin site na Lyophilized Amebocyte Lysate (TAL).A raara Bioendo na nyocha, na-emepe emepe na imepụta TAL reagent ihe karịrị afọ iri anọ.Ngwaahịa anyị na-ekpuchi usoro niile ejiri chọpụta endotoxin, nke bụ usoro gel-clot, usoro turbidimetric, na usoro chromogenic.
Oge nzipu: Jan-29-2019
