Ihe oriri na-adịghị pyrogen bụ ihe oriri na-enweghị endotoxin exogenous, gụnyere ndụmọdụ pipette na-enweghị pyrogen (igbe ntụaka), tubes ule na-enweghị pyrogen ma ọ bụ nke a na-akpọ endotoxin free glass tubes, pyrogen-free glass ampoules, endotoxin-free 96-mma microplates, na endotoxin- mmiri efu (mmiri depyrogenated eji na nje bacteria endotoxin test), endotoxin free buffer na wdg N'ime ha, mmiri maka nje endotoxintest site gel clot usoro na quantitative endotoxin test assay na niile metụtara Pharmacopoeia mbipụta (USP, EP, BP, JP). na China Pharmaceutica).Na-ezo aka na mmiri na-adịghị mma maka injection nwere ọdịnaya endotoxin na-erughị 0.015EU/ml.Ugbu a ụdị Pharmacopoeia kachasị ọhụrụ, mmiri BET erughị 0.005EU/ml.Ọbụna ọkọlọtọ kachasị elu bụ ihe na-erughị 0.001EU / ml nwere ike ịmepụta ma weta ya site na Bioendo.
Isi okwu ndị metụtara endotoxins, na ihe oriri endotoxin n'efu, pyrogen na isi mmalite okpomọkụ bụ echiche abụọ dị iche kpamkpam:Pyrogen: A na-akpọkwa pyrogen ma ọ bụ ihe exothermic.ihe ndị nwere ike ime ka mmụba ahụ dị elu.Isi iyi ọkụ: Ihe na-ebunye okpomọkụ.Dị ka ọkụ ọkụ, unyi, wdg.Ihe a na-akpọ "ndị na-abụghị pyrogenic consumables" na "nzaghachi pyrogenic" nke ụfọdụ ndị na-emepụta na ndị na-ere ahịa bụ n'ezie aha na-adịghị mma na ndị na-eduhie eduhie.Ndị ziri ezi kwesịrị ịbụ "Pyrogen Free" na "Nzaghachi Pyrogen".
Kedu ihe kpatara ihe oriri na-enweghị Pyrogen ji dị mkpa na nyocha nyocha endotoxin, ma gel clot endotoxin test assay na quantitative endotoxin test assay?
Ee, ihe oriri na-enweghị pyrogen dị mkpa maka ịme nyocha nke endotoxin n'ụzọ ziri ezi na ntụkwasị obi.Ọnụnọ nke pyrogens, bụ ihe ndị na-akpata ahụ ọkụ na-esitekarị na endotoxins nke nje bacteria, nwere ike igbochi nsonaazụ nke ule ahụ ma duga n'ịgụ ụgha.A na-eji ule endotoxin, nke a na-akpọkarị Limulus amebocyte lysate (LAL) ule ma ọ bụ nke a na-akpọ Lyophilized amebocyte lysate lysate (LAL), iji chọpụta ma chọpụta ọnụnọ nke endotoxins nke nje bacteria na ọgwụ, ngwaọrụ ahụike, na ngwaahịa ndị ọzọ.Nnwale LAL na-adabere na mmeghachi omume dị n'etiti reagent LAL na endotoxins iji mepụta nzaghachi clotting ma ọ bụ chromogenic.Iji hụ na nsonaazụ ziri ezi, ọ dị mkpa iji ihe oriri na-adịghị na pyrogens.Pyrogens nwere ike imerụ ihe ụlọ nyocha dị iche iche, gụnyereiko, ndụmọdụ pipette, tubes, na ihe nlele.Ọ bụrụ na ihe oriri ndị pyrogen mebiri emebi batara na kọntaktị LAL reagent ma ọ bụ ihe nlele, ha nwere ike ịkpalite mmeghachi omume adịgboroja, na-eduga na nkwubi okwu na-ezighi ezi banyere ọnụnọ ma ọ bụ itinye uche nke endotoxins.A na-arụpụta ihe ndị na-enweghị pyrogen na-anwale ma nwalee kpọmkwem iji belata ma ọ bụ kpochapụ ọnụnọ nke pyrogens.Ha na-enweta usoro njikwa mma siri ike iji hụ na ha ruru ụkpụrụ achọrọ maka nnwale endotoxin.Iji ihe oriri ndị a pụrụ iche na-enyere aka ịnọgide na-eguzosi ike n'ezi ihe na izi ezi nke nyocha nyocha endotoxin, na-ahụ maka nsonaazụ a pụrụ ịdabere na ya na ịkwalite nchekwa onye ọrịa na ngwa ọgwụ na ọgwụ.
Oge nzipu: Nov-07-2022
