Nyochaa Recombinant Factor C (rFC).bụ usoro a na-ejikarị achọpụta endotoxins nke nje bacteria, nke a makwaara dị ka lipopolysaccharides (LPS), Endotoxins bụ akụkụ nke mpụta nke nje bacteria Gram-negative nke nwere ike ime ka mmeghachi omume na-egbuke egbuke na ụmụ anụmanụ, gụnyere ụmụ mmadụ.Nnwale rFC dabere na iji ụdị mkpụrụ ndụ ihe nketa nke Factor C, enzyme nke a na-ahụkarị na ọbara nshịkọ akpukpọ ụkwụ ma na-etinye aka na ụzọ mkpirisi.Na nyocha nke rFC, a na-eji recombinant Factor C chọpụta ọnụnọ nke endotoxins site na nha site na ịlele ọdịnaya nke ihe ndị gbajiri agbaji na ọnụnọ endotoxin.Tụnyere usoro ọdịnala nke nchọpụta endotoxin, dị ka nyocha Limulus Amebocyte Lysate (LAL) nke na-eji ọbara nshịkọ akpukpọ inyinya, a na-ewere nyocha rFC dị ka ahaziri ahazi na imepụtagharị ya, n'ihi na ọ naghị adabere na iji reagents sitere na anụmanụ.Nyocha rFC na-enwekwa enyi na gburugburu ebe obibi ma na-adigide, ebe ọ na-ebelata mkpa maka nchịkọta na iji crabs inyinya na nchọpụta endotoxin.
Ndị isi nchịkwa kwadoro nyocha rFC, dị ka United States Pharmacopeia (USP) , European Pharmacopoeia (EP) na Chinese Pharmacopoeia (CP) maka ojiji na nyocha njikwa mma nke ọgwụ na ngwaọrụ ahụike.
Uru nke recombinant factor c assay
Nnwale Recombinant Factor C (rFC) na-enye ọtụtụ uru karịa ụzọ ọdịnala maka ịchọpụta endotoxins, dị ka nyocha Limulus Amebocyte Lysate (LAL).Ụfọdụ uru dị na nyocha rFC gụnyere:
1. Standardization: The rFC assay bụ recombinant DNA technology na-eji otu, kọwaa protein dị ka nchọpụta reagent.Nke a na-eme ka nyocha ahụ bụrụ nke ahaziri ahazi yana ọ naghị adị mfe mgbanwe ma e jiri ya tụnyere nyocha LAL, nke dabere na iji ngwakọta protein dị mgbagwoju anya ewepụtara na ọbara nshịkọ inyinya.
2. Reproducibility: Nyocha rFC nwere ọkwa dị elu nke reproducibility, ebe ọ na-eji otu protein akọwapụtara dị ka ihe nchọpụta nchọpụta.Nke a na-enye ohere maka nsonaazụ na-agbanwe agbanwe, ọbụlagodi n'ofe dị iche iche na ọtụtụ reagents.
3. Mbelata iji anụmanụ eme ihe: Nyocha rFC bụ usoro enyi na gburugburu ebe obibi na nke na-adịgide adịgide maka ịchọpụta endotoxins, n'ihi na ọ dịghị achọ iji anụmanụ dị ndụ ma ọ bụ nke a chụrụ n'àjà, dị ka crabs akpụkpọ ụkwụ.
4. Dị ọnụ ahịa: Nnwale rFC na-adịkarị ọnụ ahịa karịa nyocha LAL, n'ihi mbelata mkpa maka anụmanụ dị ndụ na ụdị nhazi nke nyocha ahụ.
5. Nkwụsi ike: Nyocha rFC siri ike ma nwee ike iji ya mee ihe n'ọtụtụ ngwa ngwa, gụnyere nyocha njikwa mma nke ọgwụ, ngwaọrụ ahụike, na ngwaahịa ndị ọzọ nwere ike ịnwe endotoxins.
6. Nkwenye nchịkwa: A kwadoro nyocha nke rFC site n'aka ndị ọrụ nchịkwa dịka United States Pharmacopeia (USP) , European Pharmacopoeia (EP) na Chinese Pharmacopoeia (CP) maka iji na-enyocha njirimara njikwa nke ọgwụ na ngwaọrụ ahụike.Nke a na-enye ntụkwasị obi dị elu na ntụkwasị obi na izi ezi nke nyocha.
Iji gboo ihe dị iche iche a na-achọ, Bioendo na-emepụta ma na-enye usoro ọdịnala nke gel clot endotoxin test assay kit, ngwa ngwa gel clot assay kit, quantitative endotoxin test assay kit gụnyere "Kinetic turbidimetric endotoxin test assay kitnakinetic chromogenic endotoxin test assay kit” .
Oge nzipu: Feb-19-2023
