Limulus amebocyte lysate (LAL) ma ọ bụ Tachypleus tridentatus lysate (TAL) bụ mkpụrụ ndụ ọbara na-amịpụta mmiri sitere na nshịkọ akpụkpọ ụkwụ.
Na endotoxins bụ ụmụ irighiri mmiri hydrophobic bụ akụkụ nke lipopolysaccharide complex nke na-etolite ọtụtụ n'ime mpụta nke nje bacteria Gram-negative.Ngwaahịa ndị nne na nna meruru na pyrogens nwere ike ibute nsonaazụ dị njọ dị ka ahụ ọkụ, ujo, ọdịda akụkụ ahụ, ma ọ bụ ọbụna ọnwụ.
LAL/TAL reagent nwere ike imeghachi omume site na nje endotoxin na lipopolysaccharide (LPS).Njikọ endotoxin nke LAL na ike imechi bụ ihe na-eme ka ọ baa uru maka ụlọ ọrụ ọgwụ nke anyị.Nke a bụ ya mere enwere ike iji LAL/TAL reagent rụọ ọrụ iji chọpụta ma ọ bụ tụọ ọnụọgụ endotoxin nje.
Tupu achọpụtara na enwere ike iji LAL/TAL mee ule endotoxins nke nje bacteria, a na-eji oke bekee were chọpụta na ọnụọgụ endotoxins dị na ngwaahịa ọgwụ.E jiri ya tụnyere RPT, nzọ na LAL / TAL reagent bụ ngwa ngwa ma rụọ ọrụ nke ọma, yana ọ bụ ụzọ a ma ama iji mee nyocha siri ike nke itinye uche endotoxin na ụlọ ọrụ ọgwụ, na ndị ọzọ.
Gel clot endotoxin test assay, nke a makwaara dị ka ule Limulus Amebocyte Lysate (LAL), ma ọ bụ nke a na-akpọ Lyophilized Amebocyte Lysate (LAL) bụ usoro a na-ejikarị eme ihe maka ịchọpụta na ịkọwapụta endotoxins na ngwaahịa dị iche iche, karịsịa na ụlọ ọrụ ọgwụ na ngwaọrụ ahụike.A na-ewere ya dị ka ihe ngwọta dị mkpa n'ọhịa nke nchọpụta endotoxin n'ihi ịdị irè ya na nnabata nchịkwa.
Nnwale LAL dabere n'ụkpụrụ na mkpụrụ ndụ ọbara nke crabs inyinya (Limulus polyphemus ma ọ bụ Tachypleus tridentatus) nwere ihe na-emekọ ihe na-emeghachi na nje endotoxins, na-eme ka e nwee mkpụkọ dị ka gel.Mmeghachi omume a na-enwe mmetụta nke ukwuu na kpọmkwem maka endotoxins, bụ ihe ndị na-egbu egbu nke akpụkpọ ahụ dị n'èzí nke nje bacteria gram-adịghị mma.
Enwere ọtụtụ ihe kpatara eji were nyocha nyocha nke gel clot endotoxin dị ka ihe ngwọta dị mkpa na nchọpụta endotoxin:
1. Nkwenye nchịkwa: A nabatara ule LAL ma nabata ya site n'aka ndị isi nchịkwa dịka United States Pharmacopeia (USP) na European Pharmacopoeia (EP) dị ka usoro ọkọlọtọ maka ule endotoxin.Irube isi na iwu ndị a bụ iwu maka ịhụ na nchekwa na ịdịmma nke ngwaahịa ọgwụ.
2. Sensitivity na Specificity: Nnwale LAL nwere mmetụta dị elu, na-enye ohere ịchọpụta ọkwa endotoxins dị ala.Ọ nwere ike ịchọpụta mkpokọta endotoxin dị obere ka nkeji 0.01 endotoxin kwa milliliter (EU/ml).Nkọwapụta nke ule ahụ na-eme ka o doo anya na ọ bụ isi na-achọpụta endotoxins ma na-ebelata nsonaazụ ụgha.
3. Ọnụ-erite uru: A na-ewerekarị gel clot endotoxin test assay dị ka ngwọta akụ na ụba ma e jiri ya tụnyere ụzọ ndị ọzọ dị ka nyocha chromogenic ma ọ bụ turbidimetric.Ọ na-achọ reagents na akụrụngwa ole na ole, na-ebelata ọnụ ahịa ule n'ozuzu ya.Na mgbakwunye, nnweta nke reagents LAL ahaziri ahazi na-eme ka ọ dabara maka ụlọ nyocha iji mee nnwale ahụ.
4. Standardkpụrụ ụlọ ọrụ: A nakweere ule LAL n'ọtụtụ ebe na ụlọ ọrụ ọgwụ na ngwaọrụ ahụike dịka usoro ọkọlọtọ maka nchọpụta endotoxin.Ọ bụ akụkụ dị mkpa nke usoro njikwa mma n'oge a na-emepụta ngwaahịa ọgwụ na ngwaọrụ ọgwụ, na-eme ka nrube isi n'usoro iwu chọrọ.
Otú ọ dị, ọ dị mkpa iburu n'obi na gel clot endotoxin test assay nwere ike inwe njedebe, dị ka nnyonye anya site na ụfọdụ ihe na ike nke ụgha-ezi ma ọ bụ ụgha-adịghị mma.N'okwu ndị akọwapụtara, enwere ike iji ụzọ ndị ọzọ dị ka nyocha chromogenic ma ọ bụ turbidimetric iji kwado ma ọ bụ kwado nsonaazụ enwetara na ule LAL.
Oge nzipu: Eprel-29-2019
