Nyocha nyocha Endotoxin nke Lyophilized Amebocyte Lysate (LAL Reagent)

Nyocha nyocha Endotoxin nke Lyophilized Amebocyte Lysate (LAL Reagent)

Ndị na-ahụ maka LALLyophilized amebocyte lysate (LAL) bụ ihe aqueous amịpụtara nke mkpụrụ ndụ ọbara (amebocytes) si Atlantic nshịkọ ịnyịnya.
TAL Reagents: TAL reagent bụ mwepụta mmiri nke mkpụrụ ndụ ọbara sitere na Tachypleus tridentatus.
Ugbu a, isi mmepụta nke LAL / TAL reagents dị na United States na China.

Gel clot endotoxin test assay, usoro a ka bụ isi ngwa n'ụwa niile, iji mezuo ihe nchekwa chọrọ nke ọgwụ injection mmadụ.
Ugbu a Bioendo na-emepụta ma na-ebunye gel clot LAL reagent gụnyere otu ampules enyo ule na vial ule ọtụtụ.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 na G52
Nke a bụ ngwọta akụ na ụba maka nchọpụta endotoxins qualitative na nyocha ọgwụ.Karịsịa maka ọgwụ injectable ma ọ bụ ọgwụ parenteral iji nwalee endotoxins na WFI, API ma ọ bụ ngwaahịa ọgwụ emechara.Nnwale ule Endotoxin bụ nnukwu ọchịchọ nke omume arụ ọrụ, chọrọ onye ọrụ ọkachamara ka ọ na-eme ihe nyocha gel clot iji hụ na ọ ga-alaghachi azụ.

A zuru ezu ngwọta makaG52Nyocha nke Endotoxin:
LAL Reagent
Control Standard Endotoxin
Nzọ mmiri
Atụmatụ pipette na-enweghị Endotoxin
Akpa iko na-enweghị Endotoxin, gụnyere ọrụ dilution na tubes mmeghachi omume.
Ngwa mgbakwunye, iji kwado bat mmiri ma ọ bụ incubator okpomọkụ akọrọ.Ngwa niile nke mmekota chọrọ ezigbo okpomọkụ.
Ihe niile eji eme ihe iji metụ nyocha LAL nke dabara na ọkwa nke enweghị endotoxin ” <0.005EU/ml.
Ebe nnwale ga-adaba na nchọpụta endotoxin.

Lezienụ anya na:
A na-eji ihe eji eme ihe plastik dị ka ndụmọdụ pipette ma ọ bụ efere multiwell maka ule endotoxin.Pharmacopoeias kwekọrọ na ya (USP/CP) chọrọ ka ihe ọ bụla plastic consumable na tubes iko ga-enwere onwe ya nke endotoxin a na-achọpụta ma ọ dịkwa mkpa mana ọ naghị echebara ya echiche, ha ga-enwerịrị ụkpụrụ nke enweghị ihe na-egbochi ya.

Kedu ka esi arụ ọrụ nyocha endotoxin iji chọpụta endotoxins dị na nlele ahụ?
Nke mbụ, a ga-eme ule maka nkwenye nke akara lysate sensitivity.Iji kwado mmetụta uche bụ otu na akara akara.
Maka nyocha nlele, mee nyocha nyocha endotoxin tupu nnyonye anya.
Iji rụọ ọrụ nyocha endotoxin zuru oke nke “nnwale nnyonye anya”.
Iji rụọ ọrụ oke ule maka inweta ka ọkwa endotoxins dị na nlele ahụ.

Mgbe ị na-arụ ọrụ nyocha maka nkwenye nke mmetụta uche lysate akpọrọ, nsonaazụ ya adịghị mma, 2 Lamada enweghị nhazi gel?
Lelee ma usoro nkwadebe nke ọkọlọtọ endotoxin na-achịkwa nje ọ̀ ziri ezi.
Achọrọ ịgwakọta Vortex maka ihe ọ bụla na-agbaze (lee ntuziaka ntuziaka ma ọ bụ na-akpọ ntinye nke ọkọlọtọ endotoxin na nkọwa).
Iji lelee ma reagent lysate dabara na ọkọlọtọ endotoxin njikwa.

A na-atụ aro ka ị na-eji mmiri ịsa ahụ ma ọ bụ ikpo ọkụ na-ekpo ọkụ na-ekpo ọkụ maka usoro okpomọkụ nke lysate reagent mgbe niile (dịka ọmụmaatụ, incubator ma ọ bụ igbe akọrọ adịghị arụ ọrụ).
N'oge incubation, mmiri gburugburu ebe obibi na mmiri ịsa ahụ bụ static na kwụsiri ike, niile vibrations na-ezere.
Lezienụ anya pụrụ iche na mmiri ịsa ahụ iji gbanyụọ mgbapụta mmiri na-asọ asọ.

Na-ekpebi nsonaazụ ahụ, jiri nwayọ wepụ tube ule ma ọ bụ ampoule iko na thermostat, jiri nwayọ tụgharịa ogo 180,
nhazi gel n'ime tube agaghị enwe nkwarụ, ọ gaghịkwa apụ apụ, e dekọrọ nsonaazụ ya site na mgbaàmà "+";
ọ dịghị gel e guzobere ma ọ bụ gel clot enweghị ike ịnọgide na-emebi emebi n'agbanyeghị na e guzobere ya.
Mgbidi mgbidi ahụ adịghị mma, e dekọrọ nsonaazụ ya site na akara "-".
Ngwa ngwa ngwa gel clot endotoxin bụ nke usoro nnwale endotoxin gel clot.
N'ime ule endotoxin gel clot ngwa ngwa, njikwa ziri ezi anaghị emepụta gel?
Nke mbụ, jiri mmiri nyocha endotoxin nke nje bacteria iji nyochaa reagents lysate, hụ na ndị ọrụ na gburugburu ebe obibi zutere ụkpụrụ;

Ọ bụrụ na kit ahụ ruru eru, mgbe ahụ njikwa dị mma nke ihe nlele ahụ anaghị emepụta gel n'ihi mmetụta mgbochi nke ihe nlele ahụ, a ga-edozikwa ihe nlele ahụ ma gwọọ ya.
Usoro nhazi ihe nlele kachasị bụ dilution.