KCET- Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay bụ ụzọ dị mkpa maka ihe nlele nwere ụfọdụ nnyonye anya.)
Nnwale kinetic chromogenic endotoxin test (KCT ma ọ bụ KCET) nyocha bụ usoro eji achọpụta ọnụnọ endotoxins na nlele.
Endotoxins bụ ihe na-egbu egbu a na-ahụ na mgbidi cell nke ụfọdụ ụdị nje bacteria, gụnyere nje bacteria gram-adịghị mma dị ka Escherichia coli na Salmonella.Na nyocha KCET, a na-agbakwunye mkpụrụ chromogenic na nlele ahụ, nke na-emeghachi omume na endotoxins ọ bụla dị ugbu a iji mepụta mgbanwe agba.
A na-enyocha ọnụego mmepe agba ka oge na-aga site na iji spectrophotometer, a na-agbakọkwa ọnụọgụ endotoxin na nlele ahụ dabere na ọnụego a.
Nyocha KCT bụ usoro ama ama maka ịchọpụta endotoxins na ọgwụ, ngwaọrụ ahụike, na ngwaahịa ndị ọzọ na-abanye na ahụ mmadụ.Ọ bụ ule dị nro na nke a pụrụ ịdabere na ya nke nwere ike ịchọpụta ọbụna obere endotoxin, na-eme ka ọ bụrụ ngwá ọrụ dị mkpa iji hụ na nchekwa nke ngwaahịa ndị a.
TAL/LAL reagent bụ lyophilized amebocyte lysate nke ewepụtara na ọbara anụnụ anụnụ nke Limulus polyphemus ma ọ bụ Tachypleus tridentatus.
Endotoxins bụ amphiphilic lipopolysaccharides (LPS) dị na mpụta cell akpụkpọ anụ nke gram-adịghị mma.Ngwaahịa ndị nne na nna meruru na pyrogens gụnyere LPS nwere ike bute mmepe nke ahụ ọkụ, ntinye nke nzaghachi mkpali, ujo, ọdịda akụkụ ahụ na ọnwụ n'ime mmadụ.
Ya mere, mba dị iche iche gburugburu ụwa ewepụtala ụkpụrụ, na-achọ ka a nwalere ngwaahịa ọgwụ ọ bụla na-ekwu na ọ bụ sterile na ndị na-abụghị pyrogenic tupu ahapụ ya.Ebu ụzọ mepụta gel-clot TAL assay maka ule endotoxins nke nje (ya bụ BET).
Agbanyeghị, ụzọ ndị ọzọ dị elu nke nyocha TAL apụtala.Ụzọ ndị a agaghị achọpụta naanị mana ọ ga-akọwapụtakwa ọnụnọ endotoxins na nlele.Ewezuga usoro gel-clot, usoro maka BET nwekwara usoro turbidimetric na usoro chromogenic.Bioendo, raara nye nchọpụta endotoxin, bụ onye nrụpụta ọkachamara iji mepụta nyocha TAL/LAL chromogenic n'ezie.
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) na-enye nha ngwa ngwa maka ọnụọgụ endotoxin.
Anyị na-enyekwa Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) yana onye na-agụ microplate ELx808IU-SN, nke nwere ike hụ na a pụrụ ịdabere na ya na arụmọrụ nke nnwale gị.
Gịnị bụ atụmatụ nkeKinetic chromogenic endotoxin test assayiji nwalee endotoxins na nlele?
The kinetic chromogenic endotoxin test assay bụ ụzọ ọzọ eji nwalee maka endotoxins na sample.Ọ nwere ọtụtụ atụmatụ:
1. Ntụle kinetic: Dị ka nyocha turbidimetric, nyocha nke kinetic chromogenic na-agụnyekwa nha nke kinetic.Ọ dabere na mmeghachi omume dị n'etiti endotoxins na mkpụrụ chromogenic iji mepụta ngwaahịa nwere agba.A na-enyocha mgbanwe nke agba agba ka oge na-aga, na-enye ohere maka ọnụọgụ endotoxin dị na nlele.
2. Nnukwu uche: The kinetic chromogenic assay na-enwe mmetụta nke ukwuu ma nwee ike ịchọpụta ọkwa dị ala nke endotoxins na nlele.Ọ nwere ike tụọ mkpokọta endotoxin n'ụzọ ziri ezi, ọbụlagodi na ọkwa dị ala, na-ahụ na nchọpụta na ọnụọgụgụ a pụrụ ịdabere na ya.
3. Ogologo ike zuru oke: Nnwale ahụ nwere oke ike dị omimi, na-enye ohere maka nha nke endotoxin n'ofe ụdị dị iche iche.Nke a pụtara na ọ nwere ike nwalee samples na ọkwa dị iche iche nke endotoxins, na-anabata ma dị ala na nke dị elu na-enweghị mkpa maka ntụle ma ọ bụ itinye uche.
4. Nsonaazụ ngwa ngwa: Nyocha chromogenic nke kinetic na-enye nsonaazụ ngwa ngwa ma e jiri ya tụnyere usoro omenala.Ọ na-enwekarị oge nyocha dị mkpụmkpụ, na-enye ohere ịnwale ngwa ngwa na nyocha nke nlele.Enwere ike nyochaa mmepe agba na oge, yana enwere ike nweta nsonaazụ ya n'ime nkeji ole na ole ruo awa ole na ole, dabere na ngwa nyocha na akụrụngwa ejiri.
5. Automation na standardization: Enwere ike ime nyocha ahụ site na iji usoro akpaaka, dị ka ndị na-agụ microplate ma ọ bụ
endotoxin-kpọmkwem analyzers.Nke a na-enye ohere maka nyocha dị elu ma na-eme ka nha nha anya na-agbanwe agbanwe na nhazi, na-ebelata njehie mmadụ na ịba ụba arụmọrụ.
6. Nkwekọrịta na ụdị ihe atụ dị iche iche: Nnwale chromogenic nke kinetic dakọtara na ụdị ụdị ụdị dị iche iche, gụnyere ọgwụ, ngwaọrụ ahụike, biologics, na mmiri mmiri.Ọ bụ ụzọ dị iche iche nwere ike itinye n'ọrụ na ụlọ ọrụ dị iche iche na ngwa ebe achọrọ nyocha endotoxin.
N'ozuzu, kinetic chromogenic endotoxin test assay na-enye usoro dị nro, ngwa ngwa na nke a pụrụ ịdabere na ya maka ịchọpụta na ịgụta ọnụ.
endotoxins na sample.A na-eji ya n'ọtụtụ ebe na ọgwụ, biotechnology, na ụlọ ọrụ nlekọta ahụike maka njikwa mma na nchekwa.
ebumnuche ntule.
Oge nzipu: Jul-29-2019
