A na-eme ule endotoxin nke nje bacteria (BET) n'ọtụtụ ụlọ nyocha ọgbara ọhụrụ n'okpuru ọnọdụ a na-achịkwa dị ka ihe dị mkpa iji zere nnyonye anya.
Kwesịrị ekwesịusoro asepticdị mkpa mgbe ị na-akwadebe na diluting ụkpụrụ na njikwa samples.Gowningomume na-abụghị nke nkịtị ụlọ nyocha ihe nchebe onwe onye (PPE) Ihe a chọrọ abụghị ihe na-echegbu ma ọ bụrụ na ngwaahịa a na-anwale na-achọ ihe nchekwa ndị nyocha akọwapụtara n'ihi nsi ma ọ bụ ọrịa.uwe akakwesịrị ịbụ TALC efu, n'ihi na TALC nwere ike ịnwe ọkwa dị ịrịba ama nke endotoxins.Ndị na-agụ efere efere, ebe ịsa mmiri, na ihe mgbochi okpomoku kpọrọ nkụA na-eji maka nnabata ihe atụ kwesịrị ịnọ n'oche ụlọ nyocha pụọ na ọwa kpo oku, ikuku ikuku, na ikuku oyi (HVAC), ịma jijiji dị egwu, na okporo ụzọ ụlọ nyocha nke nwere ike imetụta nsonaazụ ule.Oge na ọnọdụ njide sampleekwesịrị ikpebi ma mechaa dezie ya, ọ bụrụ na ọ dị mkpa, iji hụ na enwere ike iwepụta nsonaazụ ule ziri ezi na oge tozuru oke.
Dịka ọmụmaatụ, ọ bụrụ na ụlọ nyocha ahụ na-anata mmiri maka ịgba ọgwụ (WFI) ma ọ bụ ihe nlele na-arụ ọrụ, ọ ga-edobe ya na friji ma ọ bụ ọ nwere ike ịnọgide na ụlọ okpomọkụ, na ogologo oge?Tupu anwale, a na-atụ aro ka agwakọta akpa (s) nke bụ isi nke ọma tupu iwepu ule aliquot(s) maka nnwale ozugbo ma ọ bụ mgbaze na-esote.
Nnwale Bioendo Bacterial Endotoxin, nnwale ndị a gụnyeregel clot usoronyocha nke endotoxin na nyochaNnwale endotoxin nke quantitative, Gel clot method endotoxin test assay bụ qualitative endotoxin detection, ndị a nnwale chọrọ consumables bụ depyrogenation nhazi, dị ka endotoxin free mmeghachi omume tubes, dilution tubes na pyrogen free ndụmọdụ;Nchọpụta endotoxin quantitative nwere kinetic chromogenic endotoxin test, kinetic turbidimetric endotoxin test, ndị a nnwale chọrọ consumables nwere izute elu larịị nke endotoxins na-erughị.0.005EU/ml( 0.001EU / ml), dị ka endotoxin free tubes, pyrogen free ndụmọdụ, na pyrogen free microplates, ọbụna pyrogen free resevoir.Site n'ụzọ, ọ bụrụ na ọgwụgwọ sample, akpa ga-abụ endotoxin free sample karama.
N'ime ule endotoxin, nnyonye anya nwere ike ibilite site na isi mmalite dị iche iche, dị ka ihe mejupụtara matriks, ihe nleba anya ma ọ bụ akụrụngwa.
Iji zere nnyonye anya nnwale, enwere ike ịme ihe ndị a:
1. Nkwadebe nlele: Nkwadebe nke ọma dị mkpa maka nyocha endotoxin ziri ezi.
Ekwesịrị ịnwale matriks ihe nlele ahụ nke ọma ma kwalite ya iji hụ na ndakọrịta na nyocha endotoxin.
Karịsịa, ekwesịrị iwepụ ma ọ bụ belata ihe ndị na-etinye aka dị ka lipids na protein site na iji usoro kwesịrị ekwesị dị ka nzacha ma ọ bụ centrifugation.
2. Njikwa dị mma na nke na-adịghị mma: Ọ dị mkpa ịgụnye njikwa dị mma na nke na-adịghị mma na nyocha iji nyochaa maka nnyonye anya.
Njikwa dị mma na-akwado ọrụ nnwale ahụ, ebe njikwa adịghị mma na-achọpụta mmetọ ma ọ bụ nnyonye anya ọ bụla sitere na akụrụngwa nyocha.
3. Quality Control: Quality akara kwesịrị ịrụ na niile reagents, akụrụngwa, na mmiri na-eji na assay.
Nke a na-eme ka ndị reagents nweere onwe ha pụọ na mmetọ endotoxin ma na-arụ ọrụ nke ọma.
4. Standardization: The assay kwesịrị ịhazi iji hụ na nsonaazụ niile nwere ike iji tụnyere ma nwee ike imepụtaghachi.
Nke a na-agụnye iji usoro ọkọlọtọ iji mezie nyocha na iji usoro ahaziri ahazi maka nkwadebe sample, ntinye, na nchọpụta.
5. Nkwenye: Ekwesịrị ịkwado nyocha ahụ iji hụ na ọ bụ kpọmkwem, dị nro, na ntụkwasị obi.
Nke a na-agụnye ịnwale ụdị nlele dị iche iche, gụnyere ndị a maara na ha nwere endotoxin, iji chọpụta izi ezi na nkenke nke nyocha ahụ.
Site n'ịgbaso usoro ndị a, enwere ike ibelata nnyonye anya, enwere ike nweta nnwale endotoxin ziri ezi.
Oge nzipu: Dec-01-2022
