Ngwa nke Usoro Chromogenic na Nnwale Endotoxins Bacterial

Usoro Chromogenic bụ otu n'ime usoro atọ nke nwekwara usoro gel-clot na usoro turbidimetric iji chọpụta ma ọ bụ chọpụta endotoxins sitere na nje bacteria Gram-negative site na iji amoebocyte lysate ewepụtara na ọbara anụnụ anụnụ nke crab inyinya (Limulus polyphemus ma ọ bụ Tachypleus tridentatus).Enwere ike kewaa ya dị ka nyocha njedebe-chromogenic ma ọ bụ nyocha nke kinetic-chromogenic dabere na ụkpụrụ nyocha nke ejiri rụọ ọrụ.

Ụkpụrụ mmeghachi omume bụ na: amebocyte lysate nwere cascade nke serine protease enzymes (proenzymes) nke nwere ike ịgbalite site na nje endotoxins.Endotoxins na-eme ka proenzymes rụọ ọrụ iji mepụta enzymes arụ ọrụ (a na-akpọ coagulase), nke ikpeazụ na-eme ka nkewa nke mkpụrụ na-enweghị agba, na-ahapụ pNA ngwaahịa na-acha odo odo.Enwere ike tụọ pNA ewepụtara na fotometric na 405nm.A na-ejikọta nnabata ahụ nke ọma na ntinye uche nke endotoxin, mgbe ahụ enwere ike ịgbatị mkpokọta endotoxin n'otu aka ahụ.